Expression of codon-optimized TgMIC16 in three Escherichia coli strains
نویسندگان
چکیده
منابع مشابه
codon-optimized expression and purification of truncated orf2 protein of hepatitis e virus in escherichia coli
conclusions in this study, the optimized truncated orf2 protein was expressed in e. coli successfully and the highly purified protein was obtained, which can be a potential vaccine candidate and as an antigen in elisa to diagnose hev infections. results the subcloning was confirmed by pcr, restriction enzyme digestion, and dna sequencing of recombinant plasmid pet30a-orf2.2. the results obtaine...
متن کاملCodon-Optimized Expression and Purification of Truncated ORF2 Protein of Hepatitis E Virus in Escherichia coli
BACKGROUND Hepatitis E virus (HEV) is a causative agent of acute hepatitis among people of different age groups and has high mortality rate of up to 30% among pregnant women. Therefore, primary prevention of HEV infection is essential. OBJECTIVES The aim of this study was to obtain the highly purified truncated open reading frames 2 (ORF2) protein, which might be a future HEV vaccine candidat...
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Salidroside, a plant secondary metabolite in Rhodiola, has been demonstrated to have several adaptogenic properties as a medicinal herb. Due to the limitation of plant source, microbial production of salidroside by expression of plant uridine diphosphate glycosyltransferase (UGT) is promising. However, glycoside production usually remains hampered by poor expression of plant UGTs in microorgani...
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BACKGROUND Pernisine is an extracellular serine protease from the hyperthermophilic Archaeon Aeropyrum pernix K1. Low yields from the natural host and expression problems in heterologous hosts have limited the potential applications of pernisine in industry. METHODOLOGY/ PRINCIPAL FINDINGS The challenges of pernisine overexpression in Escherichia coli were overcome by codon preference optimis...
متن کاملPhylogenetic analysis of Escherichia coli strains isolated from human samples
Escherichia coli (E. coli) is a normal inhabitant of the gastrointestinal tract of vertebrates, including humans. Phylogenetic analysis has shown that E. coli is composed of four main phylogenetic groups (A, B1, B2 and D). Group A and B1 are generally associated with commensals, whereas group B2 is associated with extra-intestinal pathotypes. Most enteropathogenic isolates, however, are assigne...
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ژورنال
عنوان ژورنال: 3 Biotech
سال: 2017
ISSN: 2190-572X,2190-5738
DOI: 10.1007/s13205-017-0885-4